Bio-Plex Multiplex Immunoassay System (2024)

Table of Contents
On This Page Comparison of Bio-Plex Multiplexing vs. ELISA Bio-Plex Assays, Instruments, and Software Bio-Plex Assay Finder Bio-Plex Assays, Kits, and Reagents Bio-Plex Instruments Bio-Plex Software Bio-Plex Standard Lot Data Free 96-Well Plate Template Post-It Pad Resources Bio-Plex Multiplex Immunoassay Manuals, Guides, and Protocols Library Bio-Plex System Applications and Uses Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide (PDF 5 MB) Bio-Plex Pro Multiplex Immunoassays Brochure (PDF 4 MB) Bio-Plex Assay Manuals, Guides and Protocols Library Bio-Plex Assay and Sample Prep Success Guide (PDF 390 KB) Bio-Plex Hardware and Software Success Guide (PDF 319 KB) Application Notes Learn about drug toxicity monitoring in kidney proximal tubule chips (PDF 681 KB) Normal Physiological Levels of Human Cytokines Using Bio-Plex Pro Cytokine Assays (PDF 140 KB) Development and Validation of Bio-Plex Pro Human Chemokine Assays (PDF 284 KB) Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro RBM Apoptosis Assays (PDF 569 KB) Quantitative Bio-Plex Pro Human IgG, IgA, and IgM SARS-CoV-2 Serology Assays Using a Standard Curve (PDF 420 KB) Bio-Plex Custom Assay Development Reagents (PDF 67 KB) Bio-Plex Pro Human IgA, IgG, and IgM SARS-CoV-2 Serology Assay Performance in Freeze-Thawed and Refrigerated Serum, Plasma, and Saliva Samples (PDF 267 KB) Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit (PDF 386 KB) Correlation of SARS-CoV-2 Live Virus Neutralization Assay to Bio-Plex™ Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays (PDF 273 KB) Multiplex Immunodetection Webinar Series Applications & Technologies Multiplex Immunoassays Biomarker Analysis Related Products PrecisionAb Validated Western Blotting Antibodies Cell Lysis RT-qPCR Kits Stain-Free Western Workflow Microplate Systems Protein Sample Preparation PrimePCR Validated PCR Assays FAQs

Bio-Plex Multiplex Immunoassay System (1)

On This Page

Bio-Plex Multiplexing vs. Elisa Bio-Plex Assays, Instruments, and Software Resources Multiplex Immunodetection Webinar Series Related Products

Quantify over 500 different protein and peptide targets simultaneously in a single 12.5 µl sample using the Bio-Plex Multiplex System, powered by Luminex xMAP technology. The Bio-Plex System offers high-performance readers, industry-leading software, and sensitive assays in ready-to-use or custom configurations, enabling you to obtain high-quality data from limited samples.

Comparison of Bio-Plex Multiplexing vs. ELISA

Bio-Plex Multiplex Immunoassay System (2)

* Samples run in duplicate.
** Calculated as 2.2 hours required per plate.
*** Assumes 50 μl of sample used per well.

Bio-Plex Assays, Instruments, and Software

Bio-Plex Multiplex Immunoassay System (3)

Bio-Plex Assay Finder

Simply select your targets of interest to find the assay that meets your needs.

Find an Assay

Bio-Plex Assays, Kits, and Reagents

Bio-Plex Multiplex immunoassays use Luminex magnetic beads for the quantification of over 450 biologically relevant targets: assays for inflammation, disease, cancer, cell signaling and growth, apoptosis, toxicity, and more. Choose assays in premade and custom configurations, or develop your own assays for new targets.

Bio-Plex Instruments

Bio-Plex Readers and Tools include the Bio-Plex 200 and Bio-Plex 3D Systems, Bio-Plex Pro Wash Station, and maintenance, calibration, verification, and validation kits.

Bio-Plex Software

Bio-Plex Manager and Bio-Plex Data Pro Software make it simple to run instruments using xMAP technology and analyze multiplex data.

Bio-Plex Standard Lot Data

Download data for released standard lots for import into Bio-Plex Manager Software.

Bio-Plex Multiplex Immunoassay System (7)

  • Bio-Plex Multiplex Immunoassay System (8)

    Get answers to your questions about the Bio-Plex Multiplex Immunoassay System

    Contact an Expert

Free
96-Well Plate Template Post-It Pad

Simplifies the Bio-Plex Multiplex Assay process from start to finish.

Request Yours Now

Resources

  • Bio-Plex Multiplex Immunoassay Manuals, Guides, and Protocols Library

    Bio-Plex Multiplex Immunoassay System (9)

    Find Protocols

Bio-Plex System Applications and Uses

Watch short videos to learn how the Bio-Plex system works, how it is used in cardiovascular disease and leukemia research, biomarker identification, and citokinin expression in cancer.

  • Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide
    (PDF 5 MB)

  • Bio-Plex Pro Multiplex Immunoassays Brochure
    (PDF 4 MB)

  • Bio-Plex Assay Manuals, Guides and Protocols Library

  • Bio-Plex Assay and Sample Prep Success Guide
    (PDF 390 KB)

  • Bio-Plex Hardware and Software Success Guide
    (PDF 319 KB)

Bio-Plex Applications

  • Bench to Bedside - Learn how Bio-Plex assays and instruments are being used in cardiovascular disease and leukemia research.

  • Understanding Melanoma Biomarkers - Learn how the Bio-Plex system can help to identify early markers of melanoma in serum and blood samples from at-risk patients.

  • Understanding Cancer - Learn how Bio-Plex assays and instruments are being used to monitor cytokine expression in cancer and in response to cancer vaccines.

Bio-Plex Quick Tip Videos

  • Bio-Plex Quick Tips — Planning for Assay Success Part 1

  • Bio-Plex Quick Tips — Planning for Assay Success Part 2

  • Bio-Plex Quick Tips — Planning for Assay Success Part 3

Application Notes

  • Learn about drug toxicity monitoring in kidney proximal tubule chips
    (PDF 681 KB)

  • Normal Physiological Levels of Human Cytokines Using Bio-Plex Pro Cytokine Assays
    (PDF 140 KB)

  • Development and Validation of Bio-Plex Pro Human Chemokine Assays
    (PDF 284 KB)

  • Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro RBM Apoptosis Assays
    (PDF 569 KB)

  • Quantitative Bio-Plex Pro Human IgG, IgA, and IgM SARS-CoV-2 Serology Assays Using a Standard Curve
    (PDF 420 KB)

  • Bio-Plex Custom Assay Development Reagents
    (PDF 67 KB)

  • Bio-Plex Pro Human IgA, IgG, and IgM SARS-CoV-2 Serology Assay Performance in Freeze-Thawed and Refrigerated Serum, Plasma, and Saliva Samples
    (PDF 267 KB)

  • Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit
    (PDF 386 KB)

  • Correlation of SARS-CoV-2 Live Virus Neutralization Assay to Bio-Plex™ Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays
    (PDF 273 KB)

Multiplex Immunodetection Webinar Series

Join Bio-Rad multiplex immunodetection experts for a webinar series that explores the many factors that go into the design and execution of successful xMAP multiplex immunoassays. Not only will we cover the technology and general how to’s, we’ll also delve into the why’s to give you a deeper understanding and get you on the right path for success. By the end of this series, you’ll be a multiplexing expert.

Register to View Webinars

Applications & Technologies

  • Multiplex Immunoassays

    Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-PlexSuspension Array System.

  • Biomarker Analysis

    Learn how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.

Related Products

  • PrecisionAb Validated Western Blotting Antibodies

    These premium antibodies are lab-validated using strict testing criteria to ensure superior performance in western blotting detection.

  • Cell Lysis RT-qPCR Kits

    SingleShot Cell Lysis RT-qPCR Kits allow you to perform probe-based or SYBRGreen RT-qPCR assays directly from cultured cells without an RNA purification step.

  • Stain-Free Western Workflow

    Quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results.

  • Microplate Systems

    Absorbance microplate readers, assays, software, and accessories for a wide range of applications

  • Protein Sample Preparation

    Optimized kits, reagents, and instruments for protein extraction, cleanup, fractionation, and enrichment for downstream applications

  • PrimePCR Validated PCR Assays

    Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection for real-time PCR and Droplet Digital PCR

Bio-Plex Multiplex Immunoassay System (2024)

FAQs

What is bio-Plex assay? ›

Bio-Plex Multiplex Immunoassays allow you to simultaneously quantify up to 500 proteins, peptides, and nucleic acid targets in a single 12.5 µl sample. Assays for over 450 biologically relevant targets are available as ready-to-use premixed multiplex panels and singleplex sets or in custom-mixed panels.

What is a multiplex immunoassay? ›

In the biological sciences, a multiplex assay is a type of immunoassay that uses magnetic beads to simultaneously measure multiple analytes in a single experiment. A multiplex assay is a derivative of an ELISA using beads for binding the capture antibody.

What is the BioPlex method? ›

The BioPlex method is a technique for studying the interaction between proteins. Using molecular biology, proteins are tagged with hemagglutinin or FLAG and expressed in cultured cells.

What does BioPlex do? ›

Bio-Plex Multiplex immunoassays use Luminex magnetic beads for the quantification of over 450 biologically relevant targets: assays for inflammation, disease, cancer, cell signaling and growth, apoptosis, toxicity, and more.

What is the principle of Bioplex? ›

Bio-Plex Pro™ assays are essentially immunoassays formatted on magnetic beads. The assay principle is similar to that of a sandwich ELISA (Figure 1). Capture antibodies directed against the desired biomarker are covalently coupled to the beads. Coupled beads react with the sample containing the biomarker of interest.

How does Bio-Rad assay work? ›

The Bio-Rad Protein Assay is a dye-binding assay in which a differ- ential color change of a dye occurs in response to various concentrations of protein. 1 The absorbance maximum for an acidic solution of Coomassie® Brilliant Blue G-250 dye shifts from 465 nm to 595 nm when binding to protein occurs.

What are the benefits of Bioplex? ›

BOVOPLEX - CC (Vitamin B-Complex with liver extract injection) Primarily used as supportive therapy in conditions leading to liver dysfunction viz. liver fluke infestation, fatty degeneration of liver, in the treatment of anorexia and B complex vitamin deficiency conditions.

How does Bioplex 200 work? ›

The Bio-Plex 200 system is based on Luminex xMAP technology, uses either magnetic beads or plain polystyrene beads, and offers simultaneous measurements of up to 100 analytes in as little as 25 microliters of sample with high sensitivity and precision.

How do you use Bioplex protein? ›

1. USAGE EXAMPLE : Please add 1 LEVEL TEASPOON (3 g) Bioplex Hair Protein to 1 TUBE hair dye ( 1 tube hair dye 60 ml) . 2. USAGE EXAMPLE: Please add 1 ROUNDED TEASPOON (5g) Bioplex Hair Protein to 2 TUBES hair dye (2 tubes hair dye 2x60 =120 ml ).

What are the ingredients in BioPlex? ›

Key products include complex esters and fluoroesters, marine cell algal concentrates, natural actives, peptides, silanols, and silicone delivery systems.

What are the benefits of BioPlex bolus? ›

Reduces concerns about negative mineral interactions. Supports mineral retention and tissue reserves. Reinforces mineral status, which leads to optimal overall health, immune status and reproductive function in the animal. Is readily absorbed, stored and utilized by the animal.

What is BioPlex high seven bolus used for? ›

Supplement one bolus daily minimum for 7 days as nutritional support for wound healing, recovery from mastitis, skin disorders, and hoof health. OR as directed by a Veterinarian. Direction for Use: Bolus must be supplemented orally.

What does biofilm assay do? ›

Biofilms play a critical role in a variety of contexts, including medical device infections and environmental systems. The assay provides essential insights into the biofilm development processes, helping in the design of strategies to control or eradicate biofilms.

What does luciferase assay tell you? ›

The luciferase reporter assay allows you to study the regulatory control of a gene of interest. The presence of light means that luciferase was transcribed, and therefore the regulatory region was active.

What do biological assays test for? ›

Bioassays are methods that utilize living materials to detect substances and/or determine the potential toxicity of chemicals or contaminated matrices. They are widely used to screen for potential hazardous chemicals in contaminated soils, potable and wastewater, foods, and other materials.

What is Lowry protein assay used for? ›

The Lowry Protein Assay is a widely used method for quantifying the amount of protein in a sample. It was developed by Oliver H. Lowry and colleagues in 1951 and has since become one of the most widely used protein assays due to its high sensitivity, accuracy, and reproducibility.

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